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45 The proportion of heavy smoking, defined as greater than or equal to 25 cigarettes per day, decreased from 19.1% of smokers in 1993 to 12.1% of smokers in 2004
10 L of the extracted protein samples were mixed with 3 L of a 5X sample buffer in microcentrifugation tubes, heated to 90 o C for 5 minutes, centrifuged (2,000 x g for 1 minute) and gel electrophoresis took place at 100 Volts for 60 minutes at room temperature
Demand for sugar products skyrocketedas did the profits
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[DOI] [PubMed] [Google Scholar] Sharp BM, Beyer HS
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