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PBECs were cultured in bronchial epithelium growth medium (Lonza) in flasks coated with collagen and fibronectin for at least 3 weeks in total as described [29]

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Smoke is known to induce airway inflammation via IL-6 and IL-8 production and recruit inflammatory cells including neutrophils, however the mechanism causing the release of these cytokines has not been well described 32