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Cells were washed with cold PBS and lysed in a buffer containing 10 mmol/L Tris-HCl (pH 7.4), 50 mmol/L NaCl, 5 mmol/L ethylene diamine tetra-acetic acid (EDTA), 1% Nonidet P-40
After finishing, the writer reads it again carefully and begins to analyze the understanding of each lyric
it is toxic to virtually all of them (except one, the Carolina sphinx moth, whose fat green caterpillar has evolved a way of dealing with it)
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